Back to results
US 12,161,097 B2Granted
Non-human animals having a humanized signal-regulatory protein gene
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsAndrew J. Murphy, O. Gavin Thurston, Bindu Varghese, Cagan Gurer
Life of this patent
Estimating the term…
Abstract
Genetically modified non-human animals and methods and compositions for making and using the same are provided, wherein the genetic modification comprises a humanization of an endogenous signal-regulatory protein gene, in particular a humanization of a SIRPα gene. Genetically modified mice are described, including mice that express a human or humanized SIRPα protein from an endogenous SIRPα locus.
Claims7
A method of making a genetically modified rodent embryonic stem (ES) cell, comprising
- modifying an endogenous rodent SIRPα locus in a rodent ES cell to generate a modified SIRPα locus, wherein the modified SIRPα locus comprises a humanized SIRPα gene, wherein the humanized SIRPα gene
- (i) comprises exon 1 of a rodent SIRPα gene, exons 2, 3 and 4 of a human SIRPα gene, and exons 5, 6, 7 and 8 of the rodent SIRPα gene, and
- (ii) is operably linked to the rodent SIRPα promoter at the endogenous rodent SIRPα locus, and
- wherein the rodent is mouse or rat;
- thereby obtaining the genetically modified rodent ES cell.
2 more elements
Dependencies are inferred from the claim text.