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US 12,158,470 B2Granted
Methods and compositions for live cell analysis of intracellular ATP
AssigneeSartorius BioAnalytical Instruments, Inc.
InventorsDaniel Appledorn, Cicely Schramm, Grigory Filonov, Kirk Schroeder
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Abstract
Disclosed herein are ATP biosensor fusion proteins and their use for assaying ATP levels in cells.
Claims22
A polynucleotide comprising a nucleic acid encoding a fusion protein of genus X1-X2-X3-X4-X5, wherein:
- X1 is a FRET acceptor polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 1, and X5 is a FRET donor polypeptide consisting of the amino acid sequence of SEQ ID NO:
- 4;
- X2 and X4 are selected from the group consisting of (i) X2 is A and X4 is A;
- (ii) X2 is S and X4 is SA;
- (iii) X2 is S and X4 is A;
- (iv) X2 is absent and X4 is GA;
- (v) X2 is absent and X4 is FF;
- and (vi) X2 is T and X4 is GTSG;
- and
- X3 is an ATP binding protein consisting of the amino acid sequence of SEQ ID NO:
- 5;
- wherein binding of ATP by the ATP binding protein causes interaction of the FRET acceptor polypeptide and the FRET donor polypeptide.
10 more elements
Dependencies are inferred from the claim text.