Nanopore device and methods of biosynthesis using same

A nanopore device for synthesizing an oligonucleotide, comprising:

• a plurality of independently addressable electrodes defining a plurality of nanopore channels,
• wherein the plurality of independently addressable electrodes form an array, such that each nanopore channel of the plurality of nanopore channels is independently addressable;
• a pump to move fluid into and out of the plurality of nanopore channels;
• and
• a processor operatively coupled to the plurality of independently addressable electrodes, and the pump,
• wherein the processor is programmed to instruct the plurality of independently addressable electrodes, and the pump to perform a method, the method comprising
• a pumping a primer into a nanopore channel of the plurality of nanopore channels, the primer having a protecting group, to couple the primer to an inner wall of nanopore channel;
• b applying a voltage to an electrode of the plurality of independently addressable electrodes, to produce an acid from the electrolyte solution at the electrode;
• c the acid removing the protecting group from the primer;
• d pumping a nucleotide into the nanopore channel to couple the nucleotide to the primer with the protecting group removed to form an intermediate product;
• e repeating steps b to d on the intermediate product until the oligonucleotide is synthesized.