Cell-free protein synthesis platforms derived from clostridia extracts
AssigneesNorthwestern University, LanzaTech, Inc.
InventorsMichael Christopher Jewett, Antje Kruger-Gericke, Alexander Paul Mueller, Michael Koepke
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Abstract
Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from a species of Clostridia such as cellular extracts from Clostridium autoethanogenum.
Claims19
A cell-free protein synthesis platform for in vitro transcription of mRNA and in vitro translation of polypeptides, the platform comprising as a component a cellular extract prepared from a cell culture of a species of Clostridia, wherein the species of Clostridia is engineered to express an upregulated gene product that is a positive effector for cell-free protein synthesis (CFPS), and wherein the positive effector for CFPS is the Clostridia homolog of E. coli ackA.
A kit comprising as components:
- (a) a cellular extract prepared from a cell culture of a species of Clostridia;
- and (b) a reaction mixture for transcribing and/or translating an mRNA in the cellular extract.
A recombinant Clostridium autoethanogenum engineered to be deficient in a negative effector for cell-free protein synthesis (CFPS).
Dependencies are inferred from the claim text.