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US 12,157,901 B2Granted
Dendritic cell generator
AssigneeNortheastern University
InventorsShashi K. Murthy, Bradley B. Collier
Life of this patent
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Abstract
Devices, systems, and methods can be used for the automated production of dendritic cells (DC) from dendritic cell progenitors, such as monocytes obtained from peripheral blood. The invention makes it possible to obtain sufficient quantities of a subject's own DC for use in preparing and characterizing vaccines, for activating and characterizing the activation state of the subject's immune response, and to aid in preventing and/or treating cancer or infectious disease.
Claims20
A dendritic cell generation system comprising:
- one or more dendritic cell differentiation cassettes comprising:
- a plastic body comprising a cell culture chamber, the chamber comprising a monocyte-binding substrate;
- a fluid inlet port;
- and
- a fluid outlet port;
- wherein the plastic body, the fluid inlet port, and the fluid outlet port are fluidically coupled to the cell culture chamber and configured to provide flow of a liquid culture medium across the substrate from the inlet port to the outlet port;
- a culture medium reservoir fluidically coupled to the fluid inlet port of the one or more dendritic cell differentiation cassettes;
- a fluid collection reservoir fluidically coupled to the fluid outlet port of the one or more dendritic cell differentiation cassettes;
- and
- a pump configured for pumping fluid from the culture medium reservoir, through the cell culture chamber of the one or more dendritic cell differentiation cassettes, and into the fluid collection reservoir.
7 more elements
A method of preparing dendritic cells of a subject, the method comprising the steps of:
- providing a liquid sample comprising monocytes of the subject to a dendritic cell generation system comprising:
- one or more dendritic cell differentiation cassette comprising:
- a plastic body comprising a cell culture chamber, the chamber comprising a monocyte-binding substrate;
- a fluid inlet port;
- and
- a fluid outlet port;
- wherein the plastic body, the fluid inlet port, and the fluid outlet port are fluidically coupled to the cell culture chamber and configured to provide flow of a liquid culture medium across the substrate from the inlet port to the outlet port;
- a culture medium reservoir fluidically coupled to the fluid inlet port of the one or more dendritic cell differentiation cassettes;
- a fluid collection reservoir fluidically coupled to the fluid outlet port of the one or more dendritic cell differentiation cassettes;
- and
- a pump configured for pumping fluid from the culture medium reservoir, through the cell culture chamber of the one or more dendritic cell differentiation cassettes, and into the fluid collection reservoir;
- pumping the liquid sample into the cell culture chamber of a dendritic cell differentiation cassette of the system, whereby at least a portion of said monocytes bind to the monocyte binding substrate of the cell culture chamber;
- and
- pumping a dendritic cell differentiation medium into the cell culture chamber, whereby at least a portion of the bound monocytes differentiate into dendritic cells and detach from the monocyte-binding substrate.
11 more elements
Dependencies are inferred from the claim text.