Electroporation apparatus and method

An electroporation apparatus comprising:

• a plurality of electroporation probe terminals;
• a plurality of probe electrodes connected to said probe terminals;
• said probe electrodes being adapted for inserting into a biological load;
• a transformer for providing stepped-up voltage,
• a switching circuit with switches for linking high voltage and low voltage or ground levels to the probe terminals;
• and
• a controller configured to control said switches according to a control scheme to deliver pulses to the probe terminals, and in which said pulses are delivered to groups of at least two probe electrode terminals for said probe electrodes which are spaced apart,
• wherein said pulses include bipolar voltage pulses for a pair of electrodes;
• wherein the apparatus is configured to:
• a) deliver bipolar pulses with a frequency in the range of 100 kHz and 500 kHz with a pulse plateau duration in the range of 1 μs to 5 μs and a voltage amplitude in the range of 100 V/cm to 3000 V/cm, and
• b) deliver a monopolar pulse with a voltage in the range of 1 V/cm to 200 V/cm and a pulse duration in the range of 10 ms to 10 s.