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US 12,151,988 B2Granted
Gene targets for nitrogen fixation targeting for improving plant traits
AssigneePivot Bio, Inc.
InventorsAlvin Tamsir, Sarah Bloch, Neal Shah
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Abstract
Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in glnD, wherein said modification is selected from the group consisting of: deletion of the entire gene, deletion of substantially the entire gene, deletion of an ACT domain, deletion of more than 50% of an ACT domain, deactivation of an ACT domain, and deactivation of an UTase domain.
Claims21
A genetically engineered diazotrophic bacterium having increased expression of rpoN, wherein:
- rpoN expression is increased by deleting the native rpoN promoter and replacing it with a strong intrageneric constitutive promoter;
- and
- the genetically engineered diazotrophic bacterium is capable of increasing the amount of atmospheric derived nitrogen in a plant.
A genetically engineered diazotrophic bacterium having increased expression of rpoN, wherein:
- rpoN expression is increased by deleting the native rpoN promoter and replacing it with a strong constitutive promoter;
- and
- the genetically engineered diazotrophic bacterium is Klebsiella variicola and is capable of increasing the amount of atmospheric derived nitrogen in a plant.
Dependencies are inferred from the claim text.