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US 12,150,449 B2Granted
Extract of bacterial origin produced by the bacteria Kocuria marina sp and use thereof to generate polymer structures with bioactive surfaces able to inhibit microbial biofilm formation
OwnerAlejandro Dinamarca, Claudia Ibacache +2inventors
InventorsAlejandro Dinamarca, Claudia Ibacache, Juan Ojeda, Natalia Romo
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Abstract
The invention relates to a method for obtaining a bacterial extract produced by the bacteria Kocuria marina sp., which prevents biofilm formation, to said extract, and to the use thereof for incorporation into natural or synthetic polymers intended to form structures with active surfaces for preventing microbial biofilm formation, whether for biomedical uses, including clinical uses, industrial uses, or even domestic uses.
Claims7
A method of producing a bacterial extract that inhibits microbial biofilm formation, comprising:
- a) culturing a marine bacterium Kocuria sp strain, MM1A2AB Deposit RGM2414 strain in a sterile culture medium for marine bacteria comprising:
- between 0.75 to 1.5% w/v of a single carbon source selected from a sugar, an organic acid and an aromatic hydrocarbon and 80% (v/v) of at least one liquid selected from the group consisting of sterilized seawater and double-distilled water, wherein the at least one liquid selected from the group consisting of sterilized water and double-distilled water comprises NaCl between 3-5% w/v, so as to obtain a culture, and
- b) obtaining a supernatant of the culture obtained in a), wherein the supernatant corresponds to the bacterial extract, and comprises between 35 to 45% of palmitic acid, between 18 and 28% of stearic acid, and between 8 and 16% of oleic acid.
Dependencies are inferred from the claim text.