Glucose sensors and methods of use thereof
AssigneeBiocapital Holdings, LLC
InventorsRaul Cuero Rengifo, Laura Sanchez Londono
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Abstract
Described herein are glucose sensors. The sensors are composed of host cells incorporating DNA devices specifically designed to produce fluorescence when the cells come into contact with glucose from a patient sample. Once the fluorescence has been quantified, it can be correlated with the amount of glucose present in the sample. Also described herein are extracts from the host cells that can sense and measure glucose levels in a patient. The devices and extracts disclosed herein are inexpensive but sensitive and accurate enough for use in both home and clinical testing situations. The devices and extracts disclosed herein are also useful for diagnosis of diabetes, pre-diabetes, or other diseases associated with elevated glucose levels.
Claims34
A DNA construct comprising the following genetic components:
- (a) a gene that expresses SNF3, (b) a gene that expresses an O-linked N-acetylglucosamine transferase (OGT), (c) a gene that expresses an O-linked N-acetylglucosamine-selective-N-acetyl-β-D-glucosaminidase (OGlcNa), and (d) optionally a gene that expresses a fluorescent reporter protein, wherein
- the gene that expresses SNF3 has one of SEQ ID NO. 1 or 6-15 or at least 90% identity thereto;
- the gene that expresses O-linked N-acetylglucosamine transferase has SEQ ID NO. 2 or at least 90% identity thereto;
- and
- the gene that expresses O-linked N-acetylglucosamine-selective-N-acetyl-β-D-glucosaminidase has SEQ ID NO. 3 or at least 90% identity thereto.
Dependencies are inferred from the claim text.