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US 12,146,164 B2Granted
Compositions and methods for using small mobile stem cells
AssigneeSmsbiotech, Inc.
InventorAbdulkader Rahmo
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Abstract
Disclosed herein are small mobile stem (SMS) cells and methods of culturing, isolating, and using SMS cells. Also disclosed are methods of culturing SMS cells in an undifferentiated state for prolonged periods of time, and for using SMS cells for the production of a variety of molecules, including proteins, extracellular matrix (ECM) proteins, and the use of SMS cells in microfluidic devices.
Claims10
A method of suspension culturing a population of small mobile stem (SMS) cells, comprising:
- culturing an isolated population of SMS cells in a vessel configured to promote the growth of SMS cells in a suspension, wherein the vessel is a polypropylene vessel or a pretreated siliconized vessel containing high glucose Dulbecco's Modified Eagle Medium (DMEM) comprising calf serum, thereby maintaining the isolated population of SMS cells in suspension in an undifferentiated state,
- wherein said SMS cells are adherent equi-dimensional cells with strict radial symmetry,
- wherein said SMS cells exhibit a translucent cytoplasm, and
- wherein the SMS cells are from 4.5 to 5.5 m in diameter and are highly mobile, and
- wherein the SMS cells are derived from umbilical cord, peripheral blood, bone marrow, or solid tissue.
2 more elements
Dependencies are inferred from the claim text.