Mammalian alveolar macrophages derived from pluripotent cells
AssigneeThe Hosptial for Sick Children
InventorsMartin Post, Michael Litvack
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Abstract
Alveolar-like macrophages and a method for generating alveolar-like macrophages from hemangioblasts is provided. The method comprises the steps of: i) culturing the hemangioblasts in a hematopoietic-inducing medium comprising vascular endothelial growth factor (VEGF), stem cell factor (SCF) and interleukin-3 (IL-3) for a sufficient period of time to generate macrophages, and ii) culturing the macrophages in an alveolar macrophage-inducing medium comprising granulocyte macrophage colony stimulating factor (GM-CSF), and optionally macrophage colony stimulating factor (M-CSF), under suitable conditions and for a sufficient period of time to yield alveolar-like macrophages.
Claims6
A method of generating alveolar macrophages, the method comprising:
- i) culturing mammalian hemangioblasts in medium comprising vascular endothelial growth factor (VEGF), stem cell factor (SCF), interleukin 6 (IL6), and interleukin 3 (IL3) such that macrophages are obtained;
- ii) culturing the macrophages in medium comprising granulocyte macrophage colony stimulating factor (GM-CSF), macrophage colony stimulating factor (M-CSF), IL3, and SCF such that alveolar macrophages are obtained.
Dependencies are inferred from the claim text.