Methods for obtaining regulatory t cells and uses thereof
AssigneesInserm (Institut National De La Sante Et De La Recherche Medicale), Nantes Universite, Centre Hospitalier Universitaire De Nantes
InventorsCarole Guillonneau, Ignacio Anegon, Severine Bezie
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Abstract
Disclosed is a method for obtaining a population of human Treg cells including the steps of: (a) culturing a population of human monocytes with a medium including an amount of an interleukin-34 (IL-34) polypeptide in order to obtain a population of immunosuppressive macrophages; (b) co-culturing a population of human peripheral blood mononuclear cells (PBMCs) and the population of immunosuppressive macrophages obtained at step (a).
Claims12
A method for treating an immune and/or inflammatory disease or condition in a subject, comprising obtaining a population of human CD8+ CD45RClow Treg cells by:
- (a) culturing a population of human monocytes in presence of interleukin-34 (IL-34) polypeptide in order to obtain a population of immunosuppressive macrophages;
- (b) co-culturing a population of human peripheral blood mononuclear cells (PBMCs) and the population of immunosuppressive macrophages obtained at step (a) to obtain a population of human CD8+ CD45RClow Treg cells;
- and (c) optionally isolating the human CD8+ CD45RClow Treg cells from the population obtained at step (b);
- and administering said population or said isolated population of human CD8+ CD45RClow Treg cells to the subject, wherein the immune and/or inflammatory disease or condition is selected from the group consisting of an autoimmune disease, graft rejection and GVHD.
Dependencies are inferred from the claim text.