Methods for clonal plant production

A method of producing a transgenic plant comprising:

• providing to a plurality of haploid embryos or haploid embryo-like structures a trait gene expression cassette and a morphogenic gene expression cassette, the plurality of haploid embryos or haploid embryo-like structures comprising one or more first haploid embryos or embryo-like structures and one or more second haploid embryos or embryo-like structures, wherein at least one first haploid embryo or embryo-like structure includes the morphogenic gene expression cassette and expresses a morphogenic polypeptide;
• inducing somatic embryogenesis in the one or more second haploid embryos or embryo-like structures resulting in one or more somatic embryos, wherein somatic embryogenesis is induced by contacting the one or more second haploid embryos or embryo-like structures with the morphogenic polypeptide expressed by the at least one first haploid embryo or embryo-like structure and translocated to the one or more second haploid embryos or embryo-like structures, wherein the one or more second haploid embryos or embryo-like structures do not include the morphogenic gene expression cassette;
• selecting a somatic embryo comprising the trait gene expression cassette and lacking the morphogenic gene expression cassette;
• contacting the selected somatic embryo with a chromosome doubling agent for a period sufficient to generate a doubled haploid embryo;
• and
• regenerating a transgenic plant from the selected doubled haploid embryo, the transgenic plant comprising the trait gene expression cassette and lacking the morphogenic gene expression cassette.