PDX1 pancreatic endoderm cells in cell delivery devices and methods thereof

A cell encapsulation device, comprising:

• a first cell-excluding membrane layer and a second cell-excluding membrane layer forming a lumen therebetween configured to receive cells therein;
• a first non-woven fabric layer in contact with an external surface of the first cell-excluding membrane layer and a second non-woven fabric layer in contact with an external surface of the second cell-excluding membrane layer;
• and
• a first woven mesh layer arranged external to the first non-woven fabric layer and a second woven mesh layer arranged external to the second non-woven fabric layer, wherein the first and second cell-excluding membrane layers, the first and second non-woven fabric layers, and the first and second woven mesh layers are bonded together around their peripheries to form the cell encapsulation device.

A cell encapsulation device, comprising:

• a first cell-excluding membrane layer and a second cell-excluding membrane layer forming a lumen therebetween configured to receive cells therein;
• a first non-woven fabric layer in contact with an external surface of the first cell-excluding membrane layer and a second non-woven fabric layer in contact with an external surface of the second cell-excluding membrane layer, wherein the first and second non-woven fabric layers and the first and second cell-excluding membrane layers comprise perforations;
• and
• a port extending into the lumen and disposed between the first cell-excluding membrane layer and the second cell-excluding membrane layer, the port configured to load cells into the lumen.