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US 12,140,591 B2Granted
Compositions and methods for molecular labeling
AssigneeBio-Rad Laboratories, Inc.
InventorsMichael L. Samuels, Jeffrey Charles Olson, Andrew Watson, Keith Brown, Darren R. Link
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Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Claims13
A method of library preparation, the method comprising the steps of:
- obtaining a barcode library comprising stored barcoded oligonucleotides;
- obtaining a plurality of first constructs each comprising a first antibody coupled to a first nucleic acid sequence and a plurality second constructs each comprising a second antibody coupled to a second nucleic acid sequence, wherein each of the first constructs and each of the second constructs comprises a unique nucleic acid sequence;
- segregating the constructs in fluid compartments such that each compartment contains:
- one or more of said first constructs and/or said second constructs, and a plurality of the barcoded oligonucleotides from the barcode library;
- and
- hybridizing, after the segregating step, the first constructs and the second constructs to the barcoded oligonucleotides such that the first constructs and the second constructs in each compartment contain the same barcode.
3 more elements
Dependencies are inferred from the claim text.