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US 12,140,590 B2Granted
Compositions and methods for molecular labeling
AssigneeBio-Rad Laboratories, Inc.
InventorsMichael L. Samuels, Jeffrey Charles Olson, Andrew Watson, Keith Brown, Darren R. Link
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Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Claims10
A method for detecting and identifying target material in a fluid sample, the method comprising:
- incubating a fluid sample containing a plurality of single cells comprising target material with a plurality of antibodies conjugated to a respective plurality of target barcodes to allow the antibodies to bind to the target material;
- washing away unbound antibodies;
- isolating the single cells and cell barcodes within aqueous partitions;
- combining the target barcodes with the cell barcodes to form composite barcodes;
- releasing the composite barcodes from the antibodies;
- and
- sequencing, after the releasing step, the composite barcodes to determine sequence identity and counts of the composite barcodes, thereby quantifying the target material of the single cells.
4 more elements
Dependencies are inferred from the claim text.