Ornithine decarboxylase variant and method for producing putrescine by using same
AssigneeCj Cheiljedang Corporation
InventorsJaehun Lee, Hee-See Moon, Ae Ji Jeon, Young Lyeol Yang, Byung-Gee Kim, Eun Young Hong
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Abstract
The present application relates to a variant of ornithine decarboxylase or protein, a polynucleotide encoding the same, a microorganism containing the same, and a method for producing putrescine using the same. The present invention achieves effects of increasing putrescine productivity, production efficiency or production selectivity, suppressing side reactions, and saving the cost involved in purifying putrescine.
Claims17
An ornithine decarboxylase variant, comprising an amino acid substitution at a position corresponding to position a) 713, b) 698, or c) 713 and 698 of SEQ ID NO:
- 1, and having at least 80% but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1, wherein the substitution of the amino acid at the 713th position is A713L, A7131, A713V, A713R, A713D, A713W, or A713Q, and/or wherein the substitution of the amino acid at the 698th position is E698D.
A microorganism containing an ornithine decarboxylase variant, wherein the ornithine decarboxylase variant comprises a polypeptide of SEQ ID NO:
- 1 or a polypeptide comprising an amino acid substitution at a position corresponding to position a) 713, b) 698, or c) 713 and 698 of SEQ ID NO:
- 1 and having at least 80% but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1, wherein the substitution of the amino acid at the 713th position is A713L, A7131, A713V, A713R, A713D, A713W, or A713Q, and/or wherein the substitution of the amino acid at the 698th position is E698D.
A method for producing putrescine, comprising culturing a microorganism, wherein the microorganism comprises an ornithine decarboxylase variant, wherein the ornithine decarboxylase variant comprises a polypeptide of SEQ ID NO:
- 1, or a polypeptide comprising an amino acid substitution at a position corresponding to position a) 713, b) 698, or c) 713 and 698 of SEQ ID NO:
- 1 and having at least 80% but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1, wherein the substitution of the amino acid at the 713th position is A713L, A7131, A713V, A713R, A713D, A713W, or A713Q, and/or wherein the substitution of the amino acid at the 698th position is E698D.
A method for increasing a purity of putrescine, comprising culturing a microorganism, wherein the microorganism comprises an ornithine decarboxylase variant, wherein the ornithine decarboxylase variant comprises a polypeptide of SEQ ID NO:
- 1, or a polypeptide comprising an amino acid substitution at a position corresponding to position a) 713, b) 698, or c) 713 and 698 of SEQ ID NO:
- 1 and having at least 80% but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1, wherein the substitution of the amino acid at the 713th position is A713L, A7131, A713V, A713R, A713D, A713W, or A713Q, and/or wherein the substitution of the amino acid at the 698th position is E698D.
A method for increasing a ratio of putrescine to cadaverine (Put/Cad), comprising culturing a microorganism, wherein the microorganism comprises an ornithine decarboxylase variant, wherein the ornithine decarboxylase variant comprises a polypeptide of SEQ ID NO:
- 1, or a polypeptide comprising an amino acid substitution at a position corresponding to position a) 713, b) 698, or c) 713 and 698 of SEQ ID NO:
- 1 and having at least 80% but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1, wherein the substitution of the amino acid at the 713th position is A713L, A7131, A713V, A713R, A713D, A713W, or A713Q, and/or wherein the substitution of the amino acid at the 698th position is E698D.
A method for producing polyamide with putrescine, wherein the putrescine is produced by culturing a microorganism comprising an ornithine decarboxylase variant, wherein the ornithine decarboxylase variant comprises a polypeptide comprising an amino acid substitution at a position corresponding to position a) 713, b) 698, or c) 713 and 698 of SEQ ID NO:
- 1 and having at least 80% but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1, wherein the substitution of the amino acid at the 713th position is A713L, A713I, A713V, A713R, A713D, A713W, or A713Q and/or wherein the substitution of the amino acid at the 698th position is E698D.
A method for producing polyamide with putrescine, wherein the putrescine is produced by culturing a microorganism comprising an ornithine decarboxylase variant, wherein the ornithine decarboxylase variant comprises a polypeptide of SEQ ID NO:
- 1, or a polypeptide comprising an amino acid substitution at a position corresponding to position a) 713, b) 698, or c) 713 and 698 of SEQ ID NO:
- 1 and having at least 80% but less than 100% sequence identity to the polypeptide of SEQ ID NO:
- 1, wherein the substitution of the amino acid at the 713th position is A713L, A7131, A713V, A713R, A713D, A713W, or A713Q, and/or wherein the substitution of the amino acid at the 698th position is E698D.
Dependencies are inferred from the claim text.