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US 12,139,722 B2Granted
Methods for generating and using organoids and tissue therein
AssigneeRegents of the University of Minnesota
InventorsTimothy D. O'Brien, Beth Lindborg, Amanda Vegoe
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Abstract
This disclosure describes methods for organoid generation including, for example, for generation of a multi-tissue organoid. The multi-tissue organoid may include cartilage, bone, epithelium, and/or fibrous connective tissue. This disclosure further describes methods for isolating cells from the organoids and methods of using the organoids and cells of the organoids.
Claims15
A method comprising:
- introducing an induced pluripotent stem cell (iPSC) into a cell culture medium lacking a three-dimensional matrix, the culture medium comprising:
- a chemically defined maintenance cell culture medium, and
- hyaluronic acid in an amount effective to induce the iPSC to differentiate;
- and
- transferring the iPSC and culture medium to a non-microfluidic bioreactor, the bioreactor comprising a non-adherent culture without the use of a three-dimensional matrix;
- culturing the iPSC in the bioreactor for at least 1 week at a temperature from 32° C. to 40° C.;
- and
- producing a three-dimensional multi-tissue organoid comprising cartilage, bone, fibrous connective tissue, brain tissue, or epithelial tissue, or a combination thereof.
5 more elements
Dependencies are inferred from the claim text.