Composition for editing a nucleic acid sequence and method using the same
OwnerLung-Jr Lin, Yi-hua Hsuinventors
Life of this patent
Estimating the term…
Abstract
Provided is a composition for simultaneously targeting a nucleic acid sequence and providing intron selection in cells in vitro, ex vivo or in vivo. The composition includes one or more nucleic acid molecules each including an artificial nucleic acid sequence flanked with capping sequences, and Lambda beta protein or a linear or circular vector including a nucleic acid sequence encoding the Lambda beta protein, wherein each of the capping sequences is homologous to a region in a target nucleic acid sequence, and the artificial nucleic acid sequence is an intron sequence. The present disclosure also provides a method for editing a target nucleic acid sequence in cells by introducing the composition into the cells.
Claims16
A composition for editing a target nucleic acid sequence, comprising:
- one or more nucleic acid molecules each comprising an artificial nucleic acid sequence flanked with capping sequences, wherein each of the capping sequences is homologous to a region in the target nucleic acid sequence, wherein the nucleic acid molecule is a double-stranded DNA, and wherein the artificial nucleic acid sequence is an intron sequence comprising at least one of a splice donor site, a splice acceptor site, a branch site, a selection marker, and any combination thereof, wherein the selection marker comprises a promoter operably linked to a reporter gene to allow a selection for an integrant in genome, and wherein the artificial nucleic acid sequence is removed by RNA splicing during maturation of an RNA product of the target nucleic acid sequence;
- and
- Lambda beta protein or a linear or circular vector comprising a nucleic acid sequence encoding the Lambda beta protein.
Dependencies are inferred from the claim text.