Monoclonal antibody against canine fibroblast activation protein that cross-reacts with mouse and human fibroblast activation protein (FAP)

An isolated nucleic acid comprising a nucleic acid sequence encoding an antigen-binding domain that specifically binds to an epitope of a fibroblast activation protein (FAP), wherein the antigen binding domain comprises:

• (a) a heavy chain variable region that comprises a heavy chain complementarity determining region (HCDR) 1 comprising the amino acid sequence YTITSYSLH (SEQ ID NO:
• 1), a HCDR2 comprising the amino acid sequence EINPANGDHNFSEKFEIK (SEQ ID NO:
• 2), and a HCDR3 comprising the amino acid sequence LDDSRFHWYFDV (SEQ ID NO:
• 3);
• and
• (b) a light chain variable region that comprises a light chain complementarity determining region 1 (LCDRs) comprising the amino acid sequence TASSSVSYMY (SEQ ID NO:
• 4), a LCDR2 comprising the amino acid sequence LTSNLA (SEQ ID NO:
• 5), and a LCDR3 comprising the amino acid sequence QQWSGYPPIT (SEQ ID NO:
• 6).