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US 12,135,323 B1Granted
Serially deposited biomolecules
AssigneeCustomArray, Inc.
InventorJohn Cooper
Life of this patent
Estimating the term…
Abstract
Disclosed herein is a multiplex microarray having serially attached non-functionalized biomolecules attached to a polymer coating covering each electrode of an array of electrodes for assays and a method of making the multiplex microarray. The method comprises serially blocking the electrodes of the microarray with a blocking protein, electropolymerizing pyrrole or a functionalized pyrrole on the electrodes where the biomolecule is not present during polymerization, exposing the microarray to a biomolecular solution containing a non-functionalized biomolecule for attachment to the polymer coating, and then repeating the steps to form the multiplex microarray.
Claims20
A device comprising:
- (A) a microarray with a plurality of electronically addressable electrodes comprising at least a first microelectrode and a second microelectrode;
- (B) a plurality of first oligonucleotides, where each of the plurality of first oligonucleotides comprise a 3′ end, a 5′ end, a location, a first portion and a second portion, where the first portion extends from the 5′ end to the location, where the first portion comprises a T-linker, where the T-linker has an aminated 5′ end, where the second portion extends from the location to the 3′ end, where the first microelectrode comprises:
- a first layer consisting of a polymer formed from a monomer, where the polymer is attached to the microarray, where the first layer is formed by electropolymerizing the monomer on one or more microelectrodes of the microarray using an electropolymerizing solution with one or more electropolymerizing parameters to form a first thickness of the polymer;
- and
- two or more of the plurality of first oligonucleotides, where the T-linker of the two or more of the plurality of first oligonucleotides is immobilized on the first layer through the aminated 5′ end, where the second microelectrode comprises:
- a second layer consisting of the polymer formed from the monomer, where the polymer is attached to the microarray, where the second layer is a second thickness of the polymer, where the first thickness of the polymer differs from the second thickness of the polymer based on one or more electropolymerizing parameters selected from the group consisting of potential, current and time;
- and
- two or more of the plurality of first oligonucleotides, where the T-linker of the two or more of the plurality of first oligonucleotides is immobilized on the second layer through the aminated 5′ end.
5 more elements
A device comprising:
- (A) a microarray with a plurality of electronically addressable electrodes comprising at least a first microelectrode and a second microelectrode;
- (B) a plurality of first oligonucleotides, where each of the plurality of first oligonucleotides comprise a 3′ end, a 5′ end, a location, a first portion and a second portion, where the first portion extends from the 5′ end to the location, where the first portion comprises a T-linker, where the T-linker has an aminated 5′ end, where the second portion extends from the location to the 3′ end, where the first microelectrode comprises:
- a first layer consisting of a polymer formed from a monomer, where the polymer is attached to the microarray, where the first layer is formed by electropolymerizing the monomer on one or more microelectrodes of the microarray using an electropolymerizing solution with one or more electropolymerizing parameters to form a first thickness of the polymer;
- and
- two or more of the plurality of first oligonucleotides, where the T-linker of the two or more of the plurality of first oligonucleotides is immobilized on the first layer through the aminated 5′ end, where the second microelectrode comprises:
- a second layer consisting of the polymer formed from the monomer, where the polymer is attached to the microarray, where the second layer is a second thickness, where the first thickness of the polymer differs from the second thickness based on one or more electropolymerizing parameters selected from the group consisting of potential, current and time;
- and
- two or more of the plurality of first oligonucleotides, where the T-linker of the two or more of the plurality of first oligonucleotides is immobilized on the second layer through the aminated 5′ end;
- and
- (C) a plurality of second oligonucleotides, where the plurality of second oligonucleotides each comprise a second 3′ end, a second 5′ end, where either a cyanine moiety or a horse radish peroxidase moiety are attached to the second 5′ end, where the plurality of second oligonucleotides hybridize to the second portion.
7 more elements
A device comprising:
- (A) a microarray with a plurality of electronically addressable electrodes comprising at least a first microelectrode and a second microelectrode;
- (B) a plurality of first oligonucleotides, where each of the plurality of first oligonucleotides comprise a 3′ end, a 5′ end, a location, a first portion and a second portion, where the first portion extends from the 5′ end to the location, where the first portion comprises a T-linker, where the T-linker has an aminated 5′ end, where the second portion extends from the location to the 3′ end, where the first microelectrode comprises:
- a first layer consisting of a polymer formed from a monomer, where the monomer is selected from the group consisting of pyrrole, functionalized pyrrole, thiophenol, aniline, phenylene sulfide, phenylenediamine, diaminohaphthalene, phenol, phenolic derivative and combinations thereof, where the first layer is formed by electropolymerizing the monomer on one or more microelectrodes of the microarray using an electropolymerizing solution with one or more electropolymerizing parameters to form a first thickness;
- and
- the plurality of first oligonucleotides, where the T-linker of the two or more of the plurality of first oligonucleotides is immobilized on the first layer through the aminated 5′ end, where the second microelectrode comprises:
- a second layer consisting of the polymer formed from the monomer, where the second layer is a second thickness, where the second thickness differs from the first thickness of the polymer depends on one or more electropolymerizing parameters selected from the group consisting of potential, current and time;
- and
- the plurality of first oligonucleotides, where the T-linker of the two or more of the plurality of first oligonucleotides is immobilized on the second layer through the aminated 5′ end;
- and
- (C) a plurality of second oligonucleotides, where the plurality of second oligonucleotides each comprise a second 3′ end, a second 5′ end, where either a fluorescent moiety and a redox moiety are attached to the second 5′ end, where the plurality of second oligonucleotides hybridize to the second portion.
7 more elements
Dependencies are inferred from the claim text.