Method for analyzing metalloprotein in biological sample
AssigneeShimadzu Corporation
InventorsSatoshi Yamaki, Yun Zou
Life of this patent
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Abstract
The present invention relates to a method for analyzing a metalloprotein in a biological sample capable of continuously maintaining conditions of LC-ICPMS constant to measure a metalloprotein with high data reliability. The method for analyzing a metalloprotein which is a complex in a biological sample, the metalloprotein being a complex in which a biomolecule and a metal element bind to each other, includes: treating a biological sample which has been subjected to a pretreatment by liquid chromatography to separate the metalloprotein, and analyzing the separated metalloprotein by inductively coupled plasma mass spectrometry, wherein an ammonium acetate solution is used as a mobile phase.
Claims7
A method for analyzing a metalloprotein in a biological sample, the metalloprotein being a complex in which a biomolecule and a metal element bind to each other, the method comprising:
- treating the biological sample that has been subjected to a pretreatment by liquid chromatography to separate the metalloprotein;
- detecting the separated metalloprotein by a UV detector;
- analyzing the separated metalloprotein by inductively coupled plasma mass spectrometry after detecting the separated metalloprotein by the UV detector;
- comparing a detection signal of the UV detector with a detection signal of the inductively coupled plasma mass spectrometry;
- and
- determining that the inductively coupled plasma mass spectrometry is failed when an intensity of the detection signal of the inductively coupled plasma mass spectrometry is smaller than an intensity of the detection signal of the UV detector,
- wherein an ammonium acetate solution is used as a mobile phase.
Dependencies are inferred from the claim text.