Electrophoresis device

An electrophoresis apparatus for separation of cells in a solution, the apparatus comprising:

• a sample chamber and a harvest chamber separated by a size-exclusion membrane;
• non-circulating buffer chambers flanking each respective sample chamber and harvest chamber, wherein each buffer chamber is separated from each respective sample chamber and harvest chamber by an ion-permeable membrane that does not allow passage of macromolecules or cells, and an electrode positioned in each buffer chamber;
• wherein the ion-permeable membranes comprise poly (vinyl alcohol).