# US 12,134,778 B2 · Anti-adhesion culture medium

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- Number: US 12,134,778 B2
- Document ID: US12134778B2
- Status: Granted
- Kind: B2
- Application number: 16/766,049
- Filed: 2018-11-23
- Published: 2020-11-05
- Granted: 2024-11-05
- Earliest claimed: 2017-11-23
- Assignees: Fermentalg
- Applicants: Fermentalg
- Inventors: Philippe Boudier
- Web page: https://patentgrep.com/patent/US12134778B2

## CPC

- C12M 39/00: {Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms (apparatus for cleaning laboratory receptacles or instruments B01L13/02; cleaning in general B08B)}
- C12N 5/0018: {Culture media for cell or tissue culture (media for specific animal cell type C12N5/06)}
- C12M 21/02: {Photobioreactors (culturing algae A01G33/00, A01H4/001, C12N1/12)}
- C12N 1/10: Protozoa; Culture media therefor
- C12N 1/12: Unicellular algae; Culture media therefor (as new plants A01H13/00)
- C12N 2500/22: Zinc; Zn chelators
- C12N 2500/24: Iron; Fe chelators; Transferrin
- C12N 2533/30: Synthetic polymers
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention relates to a method for culturing microorganisms, in particular protists, carried out in such a way as to prevent the microorganisms from adhering to the walls of the reactors in which the culture is performed.

## Claims

### Claim 1

A process for culturing microorganisms in a culture medium suitable for their growth, wherein the culture medium comprises between 50 mg/L and 450 mg/L of a flocculating agent during a culture phase to prevent adhesion of the microorganisms to the walls of the reactors in which the culture is carried out, the microorganisms being microalgae of the genus Chlorella, and the flocculating agent being selected from iron chloride and zinc chloride; and wherein the culture is a long-term culture of at least 50 hours.

### Claim 2 (depends on 1)

The process according to claim 1, wherein the flocculating agent is added to the culture medium at the beginning of the culture and maintained until the end of the culture.

### Claim 3 (depends on 1)

The process according to claim 1, wherein the reactor is a carbon sink.

### Claim 4 (depends on 1)

The process according to claim 1, wherein the culture is carried out in autotrophic mode or in mixotrophic mode and the reactor comprises at least one wall that is transparent to light.

### Claim 5 (depends on 4)

The process according to claim 4, wherein the wall is polymethyl methacrylate (PMMA), polyvinyl chloride (PVC), polyethylene (PE) or polycarbonate.

### Claim 6 (depends on 1)

The process according to claim 1, wherein the flocculation efficiency is of at least 10%.

### Claim 7 (depends on 6)

The process according to claim 6, wherein the flocculation efficiency is of at least 30%.