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US 12,133,894 B2Granted
Compositions and methods for delivery of RNA
AssigneeHDT Bio Corp.
InventorsAmit Khandhar, Steven Reed, Malcolm Duthie, Jesse Erasmus, Darrick Carter, Bryan J. Berube
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Abstract
The disclosure provides nanoemulsion compositions and methods of making and using thereof to deliver a bioactive agent such as a nucleic acid to a subject. The nanoemulsion composition comprises a hydrophobic core based on inorganic nanoparticles in a lipid nanoparticle that allows imaging as well as delivering nucleic acids. Methods of using these particles for treatment and vaccination are also provided.
Claims22
A composition, wherein the composition comprises:
- DNA molecules, wherein the DNA molecules comprise a sequence encoding an antigen;
- and
- lipid nanoparticles, wherein noncomplexed lipid nanoparticles are characterized as having a z-average diameter particle size measurement of about 20 nm to about 60 nm when measured using dynamic light scattering, and wherein the lipid nanoparticles comprise:
- a surface comprising cationic lipids;
- and
- a hydrophobic core, wherein the hydrophobic core comprises liquid oil,
- wherein lipids present in the hydrophobic core are in liquid phase at 25 degrees Celsius, and
- wherein the DNA molecules are complexed to the cationic lipids to form DNA molecule-lipid nanoparticle complexes.
5 more elements
Dependencies are inferred from the claim text.