Multiplexed genome editing
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Luhan Yang, Marc Guell
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Abstract
A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.
Claims19
An isolated porcine organ comprising a plurality of genetically engineered porcine cells, wherein each genetically engineered porcine cell of said plurality is characterized by a plurality of endogenous retrovirus genes and in that at least 20% of the endogenous retrovirus genes comprise a genetically engineered genomic mutation.
An isolated porcine organ comprising a plurality of genetically engineered porcine cells, wherein each genetically engineered porcine cell of said plurality comprises genetically engineered genomic mutations in porcine endogenous retrovirus (PERV) genes and exhibits at least a 1000-fold reduction in PERV transmission co-culturing a human cell with the genetically engineered porcine cell as compared to co-culturing a human cell with a wild-type porcine cell.
Dependencies are inferred from the claim text.