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US 12,130,255 B2Granted
High-throughput label-free enzymatic bioassays using DESI-MS
AssigneePurdue Research Foundation
InventorsRobert Graham Cooks, Nicolás M. Morato, Dylan T. Holden
Life of this patent
Estimating the term…
Abstract
The invention generally relates to high-throughput label-free enzymatic bioassays using desorption electrospray ionization-mass spectrometry (DESI-MS).
Claims9
A system for analyzing one or more enzymatic reactions, the system comprising:
- a fluid handling system that includes a pinning device configured to interact with a microwell plate and prepare a plurality of discrete spots on a substrate, wherein each of the discrete spots comprises a substrate and a product of one or more enzymatic reactions, wherein both of the substrate and the product of the one or more enzymatic reactions are label-free;
- an ionization source configured to direct sequentially a discharge from the ionization source onto each of the plurality of discrete spots to sequentially desorb the substrate and/or product from each of the discrete spots and sequentially generate ions of the substrate and/or product from each of the discrete spots;
- a mass spectrometer positioned to receive the generated ions of the substrate and/or product from each of the discrete spots and analyze sequentially the ions of the substrate and/or product from each of the discrete spots in the mass spectrometer to thereby analyzing the one or more enzymatic reactions;
- and
- a single control system that comprises one or more processors that control and coordinate the fluid handling system, the ionization source, and the mass spectrometer, such that the system is a fully integrated singular system and wherein one of the one or more processors is for determining whether the test compound inhibits the enzymatic reaction based on monitoring of the progress of the enzymatic reaction.
2 more elements
A system for analyzing one or more enzymatic reactions, the system comprising:
- a fluid handling system that includes a pinning device configured to interact with a microwell plate and prepare a plurality of discrete spots on a substrate, wherein each of the discrete spots comprises a substrate and a product of one or more enzymatic reactions, wherein both of the substrate and the product of the one or more enzymatic reactions are label-free;
- an ionization source configured to direct sequentially a discharge from the ionization source onto each of the plurality of discrete spots to sequentially desorb the substrate and/or product from each of the discrete spots and sequentially generate ions of the substrate and/or product from each of the discrete spots;
- a mass spectrometer positioned to receive the generated ions of the substrate and/or product from each of the discrete spots and analyze sequentially the ions of the substrate and/or product from each of the discrete spots in the mass spectrometer to thereby analyzing the one or more enzymatic reactions;
- and
- a single control system that comprises one or more processors that control and coordinate the fluid handling system, the ionization source, and the mass spectrometer, such that the system is a fully integrated singular system wherein a subset of each of the plurality of the discrete spots further comprises an inhibitor of an enzyme of the enzymatic reaction and a test compound and wherein one of the one or more processors determines whether the test compound can counteract the inhibitor and re-start the enzymatic reaction.
2 more elements
Dependencies are inferred from the claim text.