Microorganisms and methods for the production of fatty acids and fatty acid derived products
AssigneeCargill, Incorporated
InventorsMichael D. Lynch, Michael Tai Man Louie, Shelley Copley, Eileen Colie Spindler, Brittany Robinson, Matthew Lipscomb, Tanya Lipscomb-Warnecke, Hans H. Liao, David Hogsett, Ron Evans
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Abstract
This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.
Claims19
A genetically modified organism comprising a heterologous nucleic acid sequence encoding a NphT7 polypeptide, a heterologous nucleic acid sequence encoding a thioesterase, and comprising a heterologous nucleic acid sequence encoding a modified NphT7 polypeptide;
- wherein the modified NphT7 polypeptide comprises amino acid substitutions at positions Ile147 and Phe217, and wherein said microorganism is capable of producing an acyl-CoA having a carbon chain length of C6 or greater.
Dependencies are inferred from the claim text.