Back to results
US 12,129,460 B2Granted
Methods for ethanol-free mRNA purification
AssigneeSanofi
InventorsEric Calvosa, Nicolas Chaudet, Arthur Leclercq, Alban Lepetitcolin
Life of this patent
Estimating the term…
Abstract
Provided herein are methods of purifying messenger RNA (mRNA) by subjecting a preparation comprising in vitro synthesized mRNA to one or more steps of enzymatic digestion with a proteinase, optionally with a further oligo dT affinity chromatography step. Also provided are mRNA purified by the methods described herein.
Claims21
A method of purifying messenger RNA (mRNA), comprising:
- (a) subjecting a preparation comprising in vitro synthesized mRNA to an enzymatic digestion with a proteinase;
- (b) subjecting the preparation obtained from step (a) to a step of tangential flow filtration (TFF);
- (c) subjecting the preparation obtained from step (b) to a step of capping;
- (d) subjecting the preparation obtained from step (c) to a step of frontal filtration;
- (e) subjecting the preparation obtained from step (d) to an oligo dT affinity chromatography;
- and
- (f) subjecting the preparation obtained from step (e) to a step consisting of TFF, thereby purifying the mRNA.
4 more elements
Dependencies are inferred from the claim text.