Method for producing an antitumoral arenavirus as well as arenavirus mutants
AssigneeAbalos Therapeutics GmbH
InventorsKarl Lang, Cornelia Hardt, Michael Bergerhausen, Hilal Bhat
Life of this patent
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Abstract
The invention relates to a mutant of an arenavirus having improved antitumoral properties. The invention also relates to a method of generating such an arenavirus mutant, related pharmaceutical compositions, medical uses, methods of treatment, and isolated proteins and nucleic acids.
Claims22
A mutant of lymphocytic choriomeningitis virus, wherein the mutant comprises a nucleic acid encoding a glycoprotein comprising a sequence that is at least 95% identical to residues 59-265 of SEQ ID NO:
- 10 and comprises Arg 185→Trp and Ile 181→Met amino acid substitutions as compared to the wild type glycoprotein sequence set forth in SEQ ID NO:
- 10.
An A protein comprising:
- (i) an amino acid sequence having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 26, SEQ ID NO:
- 34, SEQ ID NO:
- 42, and SEQ ID NO:
- 50, wherein the protein comprises Arg 185→Trp and Ile 181→Met amino acid substitutions as compared to the wild type glycoprotein sequence set forth in SEQ ID NO:
- 10;
- or
- (ii) an amino acid sequence having at least 95% sequence identity to the sequence of SEQ ID NO:
- 50, wherein the protein comprises an Arg 185→Trp amino acid substitution as compared to the wild type glycoprotein sequence set forth in SEQ ID NO:
- 10.
A nucleic acid, wherein the nucleic acid encodes a protein comprising an amino acid sequence
- (i) having at least 95% sequence identity to a sequence selected from the group consisting of SEQ ID NO:
- 26, SEQ ID NO:
- 34, and SEQ ID NO:
- 42, wherein the protein comprises Arg 185→Trp and Ile 181→Met amino acid substitutions as compared to the wild type glycoprotein sequence set forth in SEQ ID NO:
- 10;
- or
- (ii) having at least 95% sequence identity to the sequence of SEQ ID NO:
- 50, wherein the protein comprises an Arg 185→Trp amino acid substitution as compared to the wild type glycoprotein sequence set forth in SEQ ID NO:
- 10.
A mutant of lymphocytic choriomeningitis virus, wherein the mutant comprises a nucleic acid encoding a glycoprotein comprising a sequence that is at least 95% identical to residues 59-265 of SEQ ID NO:
- 10, wherein the protein comprises an Arg 185→Trp amino acid substitution as compared to the wild type glycoprotein sequence set forth in SEQ ID NO:
- 10.
Dependencies are inferred from the claim text.