Methods and means for the production of Ig-like molecules
AssigneeMerus N.V.
InventorsCornelis Adriaan De Kruif, Linda Johanna Aleida Hendriks, Ton Logtenberg
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Abstract
The invention provides means and methods for producing one or more Ig-like molecules in a single host cell. Novel CH3 mutations enabling the production of monospecific and/or bispecific Ig-like molecules of interest are also provided.
Claims26
A method for producing a heterodimeric antibody from a single cell, wherein said antibody comprises two heavy chains with human CH3 domains that are capable of forming an interface, said method comprising:
- providing a host cell comprising (i) a nucleic acid encoding a first antibody heavy chain comprising a positively charged residue at position 364 in the CH3 domain according to the EU numbering system, and (ii) a nucleic acid encoding a second antibody heavy chain comprising a negatively charged residue at position 368 in the CH3 domain according to the EU numbering system;
- and culturing said host cell and allowing for expression to produce said first antibody heavy chain and said second antibody heavy chain.
A method of making a host cell for production of a heterodimeric antibody, the method comprising introducing into said host cell nucleic acid molecules encoding at least a first and second antibody heavy chain, wherein the CH3 domain of said first antibody heavy chain comprises a lysine (K) residue at position 364, and the CH3 domain of the second heavy chain comprises an aspartic acid (D) residue at position 368 based on the EU numbering system, wherein said nucleic acid sequences are introduced consecutively or concomitantly.
Dependencies are inferred from the claim text.