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US 12,123,042 B2Granted
Production of steviol glycosides in recombinant hosts
AssigneeDanstar Ferment AG
InventorsHelene Lunde Robertsen, Iben Nordmark Andersen, Adam Matthew Takos, Swee Chuang Lim Hallwyl, Francesca Ambri, Manuel Quiros Asensio, Michael Dalgaard Mikkelsen, Jens Houghton-Larsen, Veronique Douchin, Jane Dannow Dyekjaer, Simon Carlsen, Nina Nicoline Rasmussen, Esben Halkjaer Hansen
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Abstract
The invention relates to recombinant microorganisms and methods for producing steviol glycosides and steviol glycoside precursors.
Claims13
A recombinant host, comprising:
- (a) a gene encoding a cytochrome P450 reductase (CPR) polypeptide;
- wherein the CPR polypeptide comprises a CPR polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:98;
- and
- (b) a gene encoding an ent-kaurenoic acid hydroxylase (KAH) polypeptide;
- wherein the KAH polypeptide comprises a KAH polypeptide having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:82;
- wherein at least one of the genes is a recombinant gene;
- wherein the recombinant host produces a steviol or a steviol glycoside precursor;
- and
- wherein the recombinant host cell is a fungal cell.
6 more elements
Dependencies are inferred from the claim text.