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US 12,123,041 B2Granted
Method for the production of amino sugar-containing products
AssigneeUniversiteit Gent
InventorsJo Maertens, David Bauwens, Wouter Van Bellegem, Pieter Coussement, Dries Duchi, Marjan De Mey
Life of this patent
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Abstract
A method or producing amino sugar (containing) products using metabolically engineered microorganisms is disclosed, wherein the conversion of UDP-N-acetylglucosamine to cell envelope precursors and molecules is reduced by altering the activity of enzymes involved in the synthesis of cell envelope precursors and molecules.
Claims2
A method of producing a UDP-N-acetylglucosamine (UDP-GlcNAc)-derived saccharide in a gram-negative bacterium, wherein the method comprises:
- a) decreasing but not inactivating expression of a murA gene in a gram-negative bacterium, wherein the expression of the murA gene is decreased by modifying a promoter or 5′-UTR region(s) of the murA gene of the gram-negative bacterium,
- b) cultivating the gram-negative bacterium with decreased murA gene expression to produce a UDP-GlcNAc-derived saccharide, and
- c) extracting and purifying UDP-GlcNAc-derived saccharide therefrom.
Dependencies are inferred from the claim text.