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US 12,123,004 B2Granted
Generation of acyl amino acids
AssigneeModular Genetics, Inc.
InventorsKevin A. Jarrell, Michelle Pynn
Life of this patent
Estimating the term…
Abstract
Engineered polypeptides or engineered microbial cells useful in synthesizing acyl amino acids are provided. In some embodiments, engineered polypeptides or engineered microbial cells are useful in synthesizing acyl amino acids with one or more hydroxyl and/or methyl groups at one or more positions of the fatty acid portion of the acyl amino acid (e.g., at ω-1, ω-2, and/or ω-3 positions of the fatty acid portion of the acyl amino acid). Also provided are methods of making acyl amino acids using engineered polypeptides and/or engineered microbial cells.
Claims14
An engineered Bacillus subtilis cell that:
- (i) expresses at least one peptide synthetase, which peptide synthetase synthesizes an acyl amino acid, and (ii) comprises a modification that comprises deletion of a fatty acid hydroxylase gene, wherein the modification results in a reduction in the number of hydroxyl groups of a fatty acid portion of the acyl amino acid, as compared to when the modification is absent.
Dependencies are inferred from the claim text.