Method for producing platelets using reciprocating stirring device

A method for producing platelets, which comprises:

• a culturing step of culturing megakaryocyte cells excluding microorganism in a culture solution in a culture vessel, wherein in the culturing step, the culture solution is unsteadily stirred with two or more stirring blades fixed to a stirring shaft directly linked to a motor, at least one stirring blade of the one or more stirring blades is attached not to be orthogonal to the stirring shaft, and at least another stirring blade of the two or more stirring blades has folded oval shape structure, the stirring blades being configured for to move reciprocally in a vertical direction, a horizontal direction, or a forward and reverse rotation direction,
• wherein a main surface of the stirring blade encounters resistance when the culture solution is being stirred,
• wherein the culture vessel is a closed bioreactor, and
• wherein physical shear stress is applied to the megakaryocyte cells by a reciprocal motion of the two or more stirring blades with vertical oscillation within a range between about 30 mm and about 40 mm and maximum blade speed in vertical movement within a range between about 100 mm/s and about 200 mm/s so that the platelets production from the megakaryocyte cells is promoted with an unsteady motion caused by the reciprocal motion as an optimum motion to produce the platelets without shaking operation.