Mini point of care gas chromatographic test strip and method to measure analytes

A method for determining the concentration of at least one analyte in a fluid sample, the method comprising:

• providing a system comprising:
• a flexible base substrate;
• an electrode pair disposed over the base substrate;
• at least one sensing chemistry responsive to at least one analyte in the sample, wherein the sensing chemistry is in electrical communication with the electrode pair;
• a first chromatographic layer disposed over the at least one sensing chemistry, wherein the at least one analyte of a plurality of analytes moves through the first chromatographic layer at a different rate relative to the movement of other analytes of the plurality of analytes;
• and wherein the first chromatographic layer is affixed to the flexible base substrate;
• and wherein the first chromatographic layer comprises at least one of silicone, sulfonic acid, proton permeable membrane or acrylic acid;
• and at least one spacing layer, the at least one spacing layer disposed upon at least a portion of the base substrate, and the at least one spacing layer disposed upon at least a portion of the electrode pair, and wherein the at least one spacing layer sensing chemistry, at least in part defines a chamber, the at least one sensing chemistry disposed within the chamber;
• measuring at least one of a voltage across the electrode pair, a resistance across the electrode pair, and a current flow across the electrode pair.