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US 12,116,631 B2Granted
Creation of libraries of droplets and related species
AssigneePresident and Fellows of Harvard College
InventorsDavid A. Weitz, Jeremy Agresti
Life of this patent
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Abstract
The present invention is generally related to systems and methods for producing a plurality of droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, the fluidic droplets may be rigidified to form rigidified droplets (e.g., gel droplets). In certain embodiments, the droplets may undergo a phase change (e.g., from rigidified droplets to fluidized droplets), as discussed more herein. In some cases, a species may be added internally to a droplet by exposing the droplet to a fluid comprising a plurality of species.
Claims19
A composition comprising
- a plurality of gel droplets capable of undergoing a phase change in response to an environmental change, wherein the gel droplets are all composed of substantially the same material;
- and
- probes within the gel droplets, wherein all of the probes within any one of the gel droplets share a common droplet-specific nucleic acid sequence and wherein the probes in different ones of the gel droplets have different nucleic acid sequences whereby the probes provide the gel droplets with droplet-specific distinguishable species of nucleic acid.
Dependencies are inferred from the claim text.