Glycerol 3-phosphate oxidase mutants, compositions, devices, kits and uses thereof

A glycerol 3-phosphate oxidase mutant comprising a sequence having at least 95% sequence identity to SEQ ID NO:

• 1, with reduced oxidase activity as compared to the glycerol 3-phosphate oxidase of SEQ ID NO:
• 1 or increased dehydrogenase activity compared to the glycerol 3-phosphate oxidase of SEQ ID NO:
• 1, or both reduced oxidase and increased dehydrogenase activity compared to the glycerol 3-phosphate oxidase of SEQ ID NO:
• 1;
• and
• wherein said glycerol 3-phosphate oxidase mutant comprises a modification at one or more amino acid positions selected from:
• (a) a position corresponding to position 104 of the amino acid sequence set forth in SEQ ID NO:
• 1, wherein the modification comprises a substitution of the amino acid residue Met with an amino acid residue selected from the group consisting of Ala, Asn, Gly, His, Ile, Phe, Pro, Tyr, and Val;
• and
• (b) a position corresponding to position 169 of the amino acid sequence set forth in SEQ ID NO:
• 1, wherein the modification comprises a substitution of the amino acid residue Asp with the amino acid residue Glu.