Extended metabolism methods for increasing and extracting metabolites from algae and microorganisms
AssigneeSynergraze Inc.
InventorsJianwei Chen, Tamara Lee Loiselle
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to methods and systems for extending metabolism of algal biomass or enzyme extracts, including the production and extraction of algal secondary metabolites with pharmaceutical, industrial, and agricultural uses. Aspects of this disclosure relate to production of antimethanogenic compounds from algae and other organisms. The disclosure further relates to compositions comprising antimethanogenic compounds and methods of using the same to reduce enteric methane emissions from ruminate animals. Methods of culturing algae in solutions with oil layers and triggering release of secondary metabolites through stress are also described.
Claims16
A method for producing an antimethanogenic halide compound, said method comprising the steps of:
- a) providing a haloperoxidase enzyme;
- b) providing a reaction solution, said reaction solution comprising:
- i) formate;
- ii) hydrogen peroxide;
- and
- iii) bromide;
- c) contacting the haloperoxidase enzyme with the reaction solution for a time period sufficient to produce an antimethanogenic halide compound, and
- d) separating the antimethanogenic halide compound from the reaction solution.
A method for producing an antimethanogenic halide compound, said method comprising the steps of:
- a) providing a bromoperoxidase enzyme;
- b) providing a reaction solution, said reaction solution comprising:
- i) formate;
- ii) hydrogen peroxide;
- and
- iii) bromide;
- c) contacting the bromoperoxidase enzyme with the reaction solution for a time period sufficient to synthesize an antimethanogenic halide compound;
- and
- d) separating the antimethanogenic halide compound from the reaction solution, wherein the antimethanogenic halide compound is selected from the group consisting of bromoform, methyl bromide, dibromomethane, and combinations thereof.
Dependencies are inferred from the claim text.