Thymic epithelial cells, exosomes derived therefrom, and methods of making and using same
AssigneeLoyola University of Chicago
InventorPhong Le
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Abstract
The present disclosure provides engineered thymic epithelial cells and cell lines, as well as extracellular vesicles derived therefrom. The disclosure also provides exosomes that display specific surface proteins, and provides methods for using said materials for treating subjects and for identifying cells.
Claims8
An isolated human thymic epithelial cell comprising at least one expression vector encoding a human Delta-like 4 gene (DLL4), wherein said expression vector is capable of expressing a Delta-like 4 protein, wherein said cell is capable of producing an exosome which displays human Delta-like 4 protein on its membrane surface, and wherein said exosome is capable of promoting T cell development from hematopoietic stem cell (HSC).
An isolated human thymic epithelial cell comprising at least one expression vector encoding a human Delta-like 4 gene (DLL4), wherein said expression vector is capable of expressing a Delta-like 4 protein, wherein said cell is capable of producing an exosome which displays human Delta-like 4 protein on its membrane surface, wherein said exosome binds to CD34POS HSCs, and wherein said exosome expresses CD7, CD127, CD62L, CXCR4, CCR7, and CCR9.
An isolated human thymic epithelial cell comprising at least one expression vector encoding a human Delta-like 4 gene (DLL4), wherein said expression vector is capable of expressing a Delta-like 4 protein, wherein said cell is capable of producing an exosome which displays human Delta-like 4 protein on its membrane surface, wherein said exosome promotes HSCs to commit and differentiate into CD7+, CD7 CD3+, immature CD4 CD8 double-positive, and mature single CD4+ or CD8+ T cells.
An isolated human thymic epithelial cell comprising at least one expression vector encoding a human Delta-like 4 gene (DLL4), wherein said expression vector is capable of expressing a Delta-like 4 protein, wherein said cell is capable of producing an exosome which displays human Delta-like 4 protein on its membrane surface, wherein said exosome expresses a human leukocyte antigen (HLA) and an antigenic peptide.
Dependencies are inferred from the claim text.