Target-primed macrophages and therapeutic uses thereof
AssigneeLundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center
InventorsLiana Chan, Scott G. Filler, Michael R. Yeaman
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Abstract
Cell populations, compositions, and methods are provided relating to target priming of macrophage cells. The macrophages, once primed or activated with a microorganism, can be used to prevent or treat infection by the microorganism. Likewise, once primed or activated by a tumor cell or tumor antigen, the macrophage cell can be used to prevent or treat tumor of the same kind. The priming can be carried out in vitro or ex vivo. The macrophages can be isolated from the subject of disease prevention or treatment.
Claims11
A method of preparing a population of target-primed macrophages, comprising culturing macrophages with a microorganism, in vitro or ex vivo, for 2 to 6 days under physiological conditions, thereby yielding the population of target-primed macrophages, wherein the macrophages have been prepared by isolating a monocyte or macrophage from a mammalian subject and differentiating or expanding the monocyte or macrophage, wherein the mammalian subject has been previously infected by or immunized with the microorganism, and wherein at least 50% of the target-primed macrophages in the population are M1-polarized macrophages.
Dependencies are inferred from the claim text.