Genetically modified reduced-browning fruit-producing plant and produced fruit thereof, and method of obtaining such
AssigneeOkanagan Specialty Fruits Inc.
InventorsJohn Armstrong, William David Lane
Life of this patent
Estimating the term…
Abstract
A genetically modified fruit-producing plant, said plant having sufficiently reduced total Polyphenol Oxidase (PPO) activity relative to a wild type of said plant to reduce browning in the fruit of said plant relative to said wild type, wherein the reduced total PPO activity results from a reduction in activity of at least two PPO isoenzymes in said plant relative to said wild type, or a cell, seed, seedling, part, tissue, cell, fruit or progeny of said plant.
Claims19
A genetically modified apple plant comprising a heterologous nucleic acid molecule comprising two or more of:
- a first nucleic acid sequence comprising at least 50 contiguous nucleotides of SEQ ID NO:18;
- a second nucleic acid sequence comprising at least 50 contiguous nucleotides of SEQ ID NO:20;
- a third nucleic acid sequence comprising at least 50 contiguous nucleotides of SEQ ID NO:22;
- and
- a fourth nucleic acid sequence comprising at least 50 contiguous nucleotides of SEQ ID NO:24;
- wherein when two or more of the first, second, third, and fourth nucleic acid sequences are present, each is capable of reducing expression of a corresponding first, second, third, or fourth polyphenol oxidase (PPO) isoenzymes, such that fruit of said apple plant has reduced browning potential relative to fruit of a wild type apple plant, and wherein the reduced browning potential results from reduction in expression of two or more genes encoding polyphenol oxidase (PPO) isoenzymes in said genetically modified apple plant compared to said wild type apple plant, and
- wherein at least one of the two or more nucleic acid sequences comprises the second or the fourth nucleic acid sequence.
A genetically modified apple plant comprising a heterologous nucleic acid molecule comprising three or more of:
- a first nucleic acid sequence comprising at least 50 contiguous nucleotides of SEQ ID NO:18;
- a second nucleic acid sequence comprising at least 50 contiguous nucleotides of SEQ ID NO:20;
- a third nucleic acid sequence comprising at least 50 contiguous nucleotides of SEQ ID NO:22;
- and
- a fourth nucleic acid sequence comprising at least 50 contiguous nucleotides of SEQ ID NO:24;
- wherein when three or more of the first, second, third, and fourth nucleic acid sequences are present, each is capable of reducing expression of a corresponding first, second, third, or fourth polyphenol oxidase (PPO) isoenzyme, such that fruit of said apple plant has reduced browning potential relative to fruit of a wild type apple plant, and wherein the reduced browning potential results from reduction in expression of three or more genes encoding polyphenol oxidase (PPO) isoenzymes in said genetically modified apple plant compared to said wild type apple plant.
Dependencies are inferred from the claim text.