Shewanella decolorationis producing tetrodotoxin and application thereof

A method of obtaining a tetrodotoxin of Shewanella decolorationis S3-4 strain deposited in the China General Microbiological Culture Collection Center (CGMCC) on 28 Mar. 2022 with the deposit number of CGMCC No. 24602,

• wherein the method comprises inoculating the Shewanella decolorationis S3-4 strain into a LB liquid culture medium and culturing at 28° C. at 200 rpm for 2-3 days, collecting cells from said culture of the Shewanella decolorationis S3-4 strain, and further fermentation culturing of said cells to obtain the tetrodotoxin, wherein the LB liquid culture medium comprises tryptone at a final concentration of 10.0 g/L, yeast extract powder at a final concentration of 5.0 g/L, and sodium chloride at a final concentration of 10.0 g/L,
• wherein the fermentation culturing comprises fermenting and culturing of the collected cells of the Shewanella decolorationis S3-4 strain, centrifuging at 4° C. at 4000×g for 30 minutes to obtain the fermentation cultured cells of the strain, ultrasonically crushing the fermentation cultured cells obtained by centrifugation after adding 0.1% methanol formate, centrifuging the resultant crushed cells at 8000 rpm for 5 minutes to obtain a supernatant, filtering the supernatant through an organic filter membrane with a pore size of 0.22 m, obtaining the tetrodotoxin, and subjecting the tetrodotoxin obtained to liquid chromatography-mass spectrometry to confirm that it is derived from the Shewanella decolorationis S3-4 strain.