antiRPS4Y1 mAb
AssigneesFondazione Irccs Ca'granda-Ospedale Maggiore Policilinico, Fondazione Luigi Villa
InventorsSilvia Spena, Flora Peyvandi
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Abstract
It forms an object of the present invention a monoclonal antibody or an antigen-binding fragment thereof, which specifically binds to RPS4Y1 protein, preferably to human RPS4Y1, recognizing an epitope comprising sequence Y1 (SEQ ID NO: 3), and/or sequence Y2 (SEQ ID NO: 4) and/or sequence Y3 (SEQ ID NO: 5) of RPS4Y1. In a further embodiment, it is here claimed a non-invasive method to identify male foetal cells within three months of gestation, said method comprising detecting the presence of RPS4Y1 protein in a maternal biological tissue sample by an antibody or an antigen-binding fragment thereof, according to the present invention.
Claims4
A non-invasive method for estimating gender of a foetus of a pregnant female within three months of gestation, said method comprising:
- (a) making available a maternal blood sample;
- (b) contacting said sample with monoclonal antibody #3 produced by the hybridoma having Deposit Designation PD22001, deposited with Interlab Cell Line Collection (ICLC), an antigen-binding fragment of monoclonal antibody #3, or an immunoconjugate of monoclonal antibody #3, that specifically recognizes RPS4Y1 protein, and
- (c) determining and/or quantifying binding of said antibody, antigen-binding fragment thereof, or immunoconjugate thereof in the sample from the subject,
- (d) estimating gender of said foetus based on said binding, wherein the presence in said sample of RPS4Y1 protein is indicative of a male foetus.
A method for determining the presence of RPS4Y1 protein in a biological specimen from a subject, comprising:
- (a) making available a biological specimen selected from the group consisting of cells, tissue, blood and saliva from the subject;
- (b) contacting said biological specimen with monoclonal antibody #3 produced by the hybridoma having Deposit Designation PD22001, deposited with Interlab Cell Line Collection (ICLC), an antigen-binding fragment of monoclonal antibody #3, or an immunoconjugate of monoclonal antibody #3, specifically recognizing RPS4Y1 protein, and
- (c) determining and/or quantifying binding of said antibody, antigen-binding fragment thereof, or immunoconjugate thereof to determine the presence of RPS4Y1 protein in the biological specimen of the subject.
Dependencies are inferred from the claim text.