Peristaltic pumping segment with check valve

A peristaltic pumping segment comprising:

• a tubing segment defining a resilient pumping volume between an upstream portion and a downstream portion of the tubing segment, the pumping volume being selectively engagable by a plunger disposed adjacent to the pumping volume of the tubing segment, wherein the pumping volume is selectively engaged by the plunger to expand the pumping volume to draw fluid flow into the pumping volume and to contract the pumping volume to conduct fluid flow from the pumping volume, wherein the downstream portion of the tubing segment is selectively engagable by an occluder disposed adjacent to the downstream portion, wherein the downstream portion is selectively engaged by the occluder to compress the downstream segment to restrict retrograde fluid flow from the downstream portion into the pumping volume during expansion of the pumping volume and to permit fluid flow from the pumping volume through the downstream portion during contraction of the pumping volume;
• and
• a check valve coupled to the upstream portion of the tubing segment, the check valve comprising a valve element in fluid communication with a valve inlet and a valve outlet, wherein the valve outlet is in fluid communication with the pumping volume, and the valve element is configured to permit fluid flow from the valve inlet to the pumping volume during expansion of the pumping volume and to prevent fluid flow from the pumping volume to the valve inlet during contraction of the pumping volume.