# US 12,112,934 B2 · Method for mass spectrometry > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 12,112,934 B2 - Document ID: US12112934B2 - Status: Granted - Kind: B2 - Application number: 17/753,036 - Filed: 2020-08-22 - Published: 2022-11-10 - Granted: 2024-10-08 - Earliest claimed: 2019-08-30 - Applicants: Dh Technologies Development Pte. Ltd. - Inventors: Yves Le Blanc - Web page: https://patentgrep.com/patent/US12112934B2 ## CPC - H01J 49/0009: {Calibration of the apparatus} - H01J 49/0036: {Step by step routines describing the handling of the data generated during a measurement} - H01J 49/0045: {characterised by the fragmentation or other specific reaction} - H01J 49/10: Ion sources; Ion guns - H01J: ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS (spark-gaps H01T; electric arc lamps H05B31/00; particle accelerators H05H) ## Abstract Before each sample of a series of batch samples is introduced into a liquid sample delivery device, an ion source device receives aqueous mobile phase solution from the liquid sample delivery device and ionizes its compounds, producing an ion beam. A tandem mass spectrometer performs a neutral loss or precursor ion scan on the ion beam to measure intensities of two or more precursor ions corresponding to a known aqueous mobile phase solution compound. Intensity measurements for each of the two or more different precursor ions are compared to previously stored intensities to determine the threshold times at which these measurements indicate orifice contamination. A threshold time is then predicted for a known compound of interest of the batch samples based on the m/z value of the known compound of interest and the m/z value and the threshold time of each of the two or more different precursor ions. ## Claims ### Claim 1 Apparatus for predicting during a batch sample analysis when a measurement of a known compound of interest will be affected by mass spectrometer orifice contamination, comprising: an ion source device that, before each sample of a series of batch samples is introduced into a liquid sample delivery device, receives aqueous mobile phase solution from the liquid sample delivery device and ionizes compounds of the aqueous mobile phase solution, producing an ion beam of aqueous mobile phase solution compounds; and a tandem mass spectrometer that, before each sample of the series of batch samples is introduced into the liquid sample delivery device, receives the ion beam of aqueous mobile phase solution compounds from the ion source device, performs a neutral loss or precursor ion scan to measure intensities of two or more precursor ions with different mass-to-charge ratio (m/z) values corresponding to a known aqueous mobile phase solution compound of the ion beam, producing an intensity measurement for each of the two or more different precursor ions, stores the measured intensity and time for each of the two or more different precursor ions in a memory device, compares the measured intensity of each of the two or more different precursor ions with a previously measured intensity stored in the memory device until a threshold time is found for each of the two or more different precursor ions where a measured intensity of each of the two or more different precursor ions is reduced below a threshold intensity due to contamination of an orifice of the tandem mass spectrometer, and predicting a time when an intensity of a known compound of interest of the batch samples is reduced due to contamination of the orifice based on an m/z value of the known compound of interest and an m/z value and the threshold time of each of the two or more different precursor ions. ### Claim 2 (depends on 1) The apparatus of claim 1, further comprising a display device, wherein the tandem mass spectrometer displays on the display device the time when the intensity of the known compound of interest is reduced due to contamination of the orifice. ### Claim 3 (depends on 2) The apparatus of claim 2, wherein the tandem mass spectrometer further displays on the display device a warning to clean the tandem mass spectrometer before the time when the intensity of the known compound of interest is reduced due to contamination of the orifice. ### Claim 4 (depends on 1) The apparatus of claim 1, wherein, before each sample of the series of batch samples is introduced into the liquid sample delivery device, the tandem mass spectrometer performs the neutral loss or precursor ion scan at two or more time periods until a rate of change of each measured intensity of the two or more different precursor ions over the two or more time periods decreases below a threshold rate of change. ### Claim 5 (depends on 4) The apparatus of claim 4, wherein, when the rate of change of each measured intensity of the two or more different precursor ions over the two or more time periods decreases below the threshold rate of change rate of change, the tandem mass spectrometer stores a measured intensity and time from the latest of the two or more time periods for each of the two or more different precursor ions in a memory device. ### Claim 6 (depends on 5) The apparatus of claim 5, wherein, when the rate of change of each measured intensity of the two or more different precursor ions over the two or more time periods decreases below the threshold rate of change rate of change, the tandem mass spectrometer compares a measured intensity from the latest of the two or more time periods for each of the two or more different precursor ions with a previously measured intensity stored in the memory device until a threshold time is found for each of the two or more different precursor ions where a measured intensity of each of the two or more different precursor ions is reduced below the threshold intensity due to contamination of the orifice. ### Claim 7 (depends on 1) The apparatus of claim 1, wherein the aqueous mobile phase solution compound comprises a dimer, trimer, or tetramer of a known solvent. ### Claim 8 (depends on 1) The apparatus of claim 1, wherein the known aqueous mobile phase solution compound comprises a known solvent. ### Claim 9 (depends on 8) The apparatus of claim 8, wherein the known solvent comprises methanol. ### Claim 10 (depends on 8) The apparatus of claim 8, wherein the known solvent comprises acetonitrile. ### Claim 11 (depends on 8) The apparatus of claim 8, wherein the known solvent comprises one of isopropyl alcohol (IPA) or acetone. ### Claim 12 (depends on 1) The apparatus of claim 1, wherein the known aqueous mobile phase solution compound comprises a known mobile phase additive. ### Claim 13 (depends on 12) The apparatus of claim 12, wherein the known mobile phase additive comprises one of, formic acid, acetic acid, and ammonium formate. ### Claim 14 A method for predicting during a batch sample analysis when a measurement of a known compound of interest will be affected by mass spectrometer orifice contamination, comprising: instructing an ion source device, before each sample of a series of batch samples is introduced into a liquid sample delivery device, to receive aqueous mobile phase solution from the liquid sample delivery device and to ionize compounds of the aqueous mobile phase solution using a processor, producing an ion beam of aqueous mobile phase solution compounds; instructing a tandem mass spectrometer, before each sample of the series of batch samples is introduced into the liquid sample delivery device, to receive the ion beam of aqueous mobile phase solution compounds from the ion source device, to perform a neutral loss or precursor ion scan to measure intensities of two or more precursor ions with different mass-to-charge ratio (m/z) values corresponding to a known aqueous mobile phase solution compound of the ion beam, producing an intensity measurement for each of the two or more different precursor ions and to store the measured intensity and time for each of the two or more different precursor ions in a memory device; comparing the measured intensity of each of the two or more different precursor ions with a previously measured intensity stored in the memory device until a threshold time is found for each of the two or more different precursor ions where a measured intensity of each of the two or more different precursor ions is reduced below a threshold intensity due to contamination of an orifice of the tandem mass spectrometer; and predicting a time when an intensity of a known compound of interest of the batch samples is reduced due to contamination of the orifice based on an m/z value of the known compound of interest and an m/z value and the threshold time of each of the two or more different precursor ions using the processor. ### Claim 15 A computer program product, comprising a non-transitory and tangible computer-readable storage medium whose contents include a program with instructions being executed on a processor to perform a method for predicting during a batch sample analysis when a measurement of a known compound of interest will be affected by mass spectrometer orifice contamination, the method comprising: providing a system, wherein the system comprises one or more distinct software modules, and wherein the distinct software modules comprise a control module and an analysis module; instructing an ion source device, before each sample of a series of batch samples is introduced into a liquid sample delivery device, to receive aqueous mobile phase solution from the liquid sample delivery device and to ionize compounds of the aqueous mobile phase solution using the control module, producing an ion beam of aqueous mobile phase solution compounds; instructing a tandem mass spectrometer, before each sample of the series of batch samples is introduced into the liquid sample delivery device, to receive the ion beam of aqueous mobile phase solution compounds from the ion source device, to perform a neutral loss or precursor ion scan to measure intensities of two or more precursor ions with different mass-to-charge ratio (m/z) values corresponding to a known aqueous mobile phase solution compound of the ion beam, producing an intensity measurement for each of the two or more different precursor ions, and to store the measured intensity and time for each of the two or more different precursor ions in a memory device using the control module; comparing the measured intensity of each of the two or more different precursor ions with a previously measured intensity stored in the memory device until a threshold time is found for each of the two or more different precursor ions where a measured intensity of each of the two or more different precursor ions is reduced below a threshold intensity due to contamination of an orifice of the tandem mass spectrometer using the analysis module; and predicting a time when an intensity of a known compound of interest of the batch samples is reduced due to contamination of the orifice based on an m/z value of the known compound of interest and an m/z value and the threshold time of each of the two or more different precursor ions using the analysis module.