# US 12,111,317 B2 · Flow cytometry-based platform for the detection, enumeration, and isolation of disseminated tumor cells in bone marrow aspirates

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- Number: US 12,111,317 B2
- Document ID: US12111317B2
- Status: Granted
- Kind: B2
- Application number: 16/620,580
- Filed: 2018-06-08
- Published: 2020-05-28
- Granted: 2024-10-08
- Earliest claimed: 2017-06-09
- Assignees: The Trustees of the University of Pennsylvania
- Applicants: The Trustees of the University of Pennsylvania
- Inventors: Lewis Chodosh; Elizabeth Chislock
- Web page: https://patentgrep.com/patent/US12111317B2

## CPC

- G01N 33/57515: of the breast
- A61K 49/0058: {Antibodies}
- C07K 16/32: against translation products of oncogenes
- G01N 15/10: Investigating individual particles
- G01N 15/1459: {the analysis being performed on a sample stream}
- G01N 15/01: specially adapted for biological cells, e.g. blood cells (investigating sedimentation of particle suspensions in blood G01N15/05)
- G01N 2015/1006: {for cytology}
- G01N 2800/50: Determining the risk of developing a disease
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention provides compositions and methods for detecting and isolating disseminated tumor cells (DTCs) from breast cancer in a bone marrow aspirate of a subject. The invention further provides methods of treating breast cancer in the subject.

## Claims

### Claim 1

A method of treating or preventing progression of breast cancer in a subject in need thereof, the method comprising the steps of isolating a sample from the subject, contacting the isolated sample with at least one labeled molecule that specifically binds to the cell surface marker CD49C in the sample, detecting binding of the at least one labeled molecule to the cell surface marker CD49C on a disseminated tumor cell (DTC) in the sample using flow cytometry, and administering to the subject an effective amount of a therapeutic agent for the treatment of breast cancer or prevention of breast cancer progression.

### Claim 2 (depends on 1)

The method of claim 1, wherein the sample comprises bone marrow cells.