# US 12,111,316 B2 · Reference cassette for fluorescence immunoassay diagnostic device

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- Number: US 12,111,316 B2
- Document ID: US12111316B2
- Status: Granted
- Kind: B2
- Application number: 17/418,817
- Filed: 2019-12-10
- Published: 2022-03-10
- Granted: 2024-10-08
- Earliest claimed: 2018-12-28
- Assignees: Precision Biosensor Inc.
- Applicants: Precision Biosensor Inc.
- Inventors: Hee Jun Kim; Sung Joon Kim
- Web page: https://patentgrep.com/patent/US12111316B2

## CPC

- G01N 33/54388: {based on lateral flow}
- G01N 21/6447: {by visual observation}
- G01N 21/645: {Specially adapted constructive features of fluorimeters}
- G01N 21/8483: {Investigating reagent band (test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04)}
- G01N 2021/6482: {Sample cells, cuvettes}
- G01N 2021/7759: {Dipstick; Test strip}
- G01N 2021/7786: {Fluorescence}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

Provided in the present invention is a reference cassette for a fluorescence immunoassay diagnostic device, the cassette having, arranged in a case, fluorescent glass capable of maintaining fluorescence characteristics to be constant without causing degeneration, so as to accurately provide reference measurement information about the fluorescence detection amount during testing of the measurement accuracy of the fluorescence detection of the immunoassay diagnostic device, so that testing accuracy can improve, preventing decrease in measurement accuracy and being semi-permanently usable since the denaturation or deterioration of the fluorescent glass does not occur even if the cassette is used continuously, and providing a mask having a slit so as to limit the area of the fluorescent glass to be externally exposed to a specific area, thereby generating the type of fluorescence that is the same as that of a reaction area of a sample strip to be actually used, and thus more accurate reference measurement information can be provided.

## Claims

### Claim 1

A reference cassette for a fluorescence immunoassay diagnostic apparatus which is inserted into the apparatus diagnosing a disease from a biopsy specimen using fluorescence and generates fluorescence by a test light of an optical module, the reference cassette for the fluorescence immunoassay diagnostic apparatus comprising:
a case configured to be insertable into the fluorescence immunoassay diagnostic apparatus, wherein an opening is formed at at least a part of an upper side of the case so that an inner space of the case is exposed to an outside of the case; and
a fluorescence glass disposed at the inner space of the case so that the fluorescence glass is exposed to the outside of the case through the opening of the case,
wherein the fluorescence glass is configured to generate the fluorescence by the test light of the optical module and constantly maintain fluorescence characteristics,
wherein a mask is disposed on an upper surface of the fluorescence glass inside the case so that an area of the fluorescence glass exposed to the outside of the case is limited to a specific area of the fluorescence glass,
wherein the mask has one or more slits, and the fluorescence glass is exposed through the slits,
wherein a separate fixing guide having a plurality of insertion coupling holes is coupled to an inside of the case, the fluorescence glass includes a plurality of pieces of the fluorescence glass, each piece of the fluorescence glass is insertedly coupled to a respective insertion coupling hole of the fixing guide, and the mask is coupled to the fixing guide so that each of the slits limits an outside exposure area of the fluorescence glass to a slit area, and
wherein an upper surface of the fluorescence glass and an upper surface of the fixing guide are in a same plane as each other in a state that the fluorescence glass is insertedly coupled to the insertion coupling holes of the fixing guide, and the mask is coupled to the fixing guide to contact the upper surface of the fluorescence glass and the upper surface of the fixing guide.

### Claim 2 (depends on 1)

The reference cassette for the fluorescence immunoassay diagnostic apparatus of claim 1, wherein a guide protrusion is formed at the fixing guide to guide a coupling location of the mask, and a guide hole is formed at the mask to be insertedly coupled with the guide protrusion.

### Claim 3 (depends on 1)

The reference cassette for the fluorescence immunoassay diagnostic apparatus of claim 1, wherein the slits of the mask and the fluorescence glass cross the opening of the case in one direction in a state that the slits of the mask and the fluorescence glass are arranged inside the case by the fixing guide.