# US 12,111,307 B2 · Method of assessing aging of erythrocytes

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- Number: US 12,111,307 B2
- Document ID: US12111307B2
- Status: Granted
- Kind: B2
- Application number: 16/973,172
- Filed: 2019-06-10
- Published: 2021-08-19
- Granted: 2024-10-08
- Earliest claimed: 2018-06-15
- Assignees: Institute of Rheological Functions of Food
- Applicants: Institute of Rheological Functions of Food
- Inventors: Takehiko Fujino; Shiro Mawatari; Tsunemichi Kawa; Tetsuji Koyama; Toyoharu Yamashita
- Web page: https://patentgrep.com/patent/US12111307B2

## CPC

- G01N 15/0618: {of the filter type (G01N15/0643 takes precedence)}
- G01N 33/491: {by separating the blood components (G01N15/05 takes precedence)}
- G01N 15/10: Investigating individual particles
- G01N 33/4915: {using flow cells (flow cytometry G01N15/14)}
- G01N 2015/012: {Red blood cells}
- G01N 2015/1006: {for cytology}
- G01N 2015/1028: {Sorting particles}
- G01N 2800/7042: Aging, e.g. cellular aging
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A method for assessing aging of erythrocytes that includes preparing an erythrocyte suspension from a blood sample; allowing the erythrocyte suspension to pass through a first filter, separating aged erythrocytes that do not pass through the first filter, and non-aged erythrocytes that pass through the first filter, and to calculate a deformability of erythrocytes, allowing the separated non-aged erythrocyte suspension to pass through a second filter having micropores which diameter is smaller than the first filter, separating mild aged erythrocytes that do not pass through the second filter and juvenile erythrocytes that pass through the second filter, and to calculate a deformability of the non-aged erythrocytes; and assessing aging of erythrocytes, using the deformability of erythrocyte and the deformability of non-aged erythrocyte.

## Claims

### Claim 1

A method for assessing aging of erythrocytes by using at least two types of filters having a plurality of micropores, the method comprising:
preparing an erythrocyte suspension from a blood sample;
allowing the erythrocyte suspension to pass through a first filter having a diameter of micropores of 5.50 to 8.00 μm, separating aged erythrocytes that do not pass through the first filter and non-aged erythrocytes that pass through the first filter, to calculate a first deformability which is an index showing the ability of erythrocytes contained in the erythrocyte suspension to pass through the first filter;
allowing the separated non-aged erythrocyte suspension having passed through the first filter to pass through a second filter having micropores of which diameter is 3.00 to 6.00 μm, and smaller than the diameter of micropores of the first filter, separating mild aged erythrocytes that do not pass through the second filter and juvenile erythrocytes that pass through the second filter, to calculate a second deformability which is an index showing the ability of the non-aged erythrocytes contained in the non-aged erythrocyte suspension to pass through the second filter; and
assessing aging of erythrocytes based on a size of the first deformability of erythrocytes contained in the erythrocyte suspension, and the second deformability of the non-aged erythrocytes.

### Claim 2 (depends on 1)

The method for assessing aging of erythrocytes according to claim 1, wherein a passing ratio of erythrocytes of the first filter and/or a passing ratio of erythrocytes of the second filter is further calculated, to use the passing ratio for the assessment.