# US 12,111,259 B2 · Detection kit and method for detecting abused drugs

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- Number: US 12,111,259 B2
- Document ID: US12111259B2
- Status: Granted
- Kind: B2
- Application number: 17/580,571
- Filed: 2022-01-20
- Published: 2023-03-09
- Granted: 2024-10-08
- Earliest claimed: 2021-08-26
- Assignees: National Taiwan University; Investigation Bureau, Ministry of Justice
- Applicants: National Taiwan University; Investigation Bureau, Ministry of Justice
- Inventors: Huan-Tsung Chang; Yao-Te Yen; Yin-Jue Chang; Yuh-Lin Liu
- Web page: https://patentgrep.com/patent/US12111259B2

## CPC

- G01N 21/6428: {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" (in vivo A61B5/00; immunoassay G01N33/53)}
- G01N 21/65: Raman scattering
- G01N 21/643: {non-biological material}
- G01N 33/542: with steric inhibition or signal modification, e.g. fluorescent quenching
- G01N 33/54388: {based on lateral flow}
- G01N 33/94: involving narcotics {or drugs or pharmaceuticals, neurotransmitters or associated receptors}
- G01N 2021/6432: {Quenching}
- G01N 2021/7786: {Fluorescence}
- G01N 2201/0221: Portable; cableless; compact; hand-held
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A detection kit suitable for detecting a target in a sample is provided. The detection kit includes a syringe, a first reaction container, a second reaction container, and a plurality of fluorescent substances. The syringe is loaded with first organic solvent. The first reaction container is connected to the syringe and is loaded with the sample. The second reaction container is connected to the first reaction container and is loaded with second organic solvent. The fluorescent substances are dispersed in the second organic solvent and emit fluorescence. When the target in the sample is dissolved in the first organic solvent and reacts with the fluorescent substances in the second organic solvent, the fluorescence emitted by the fluorescent substances is quenched.

## Claims

### Claim 1

A detection kit, suitable for detecting a target in a sample, comprising:
a syringe, loaded with first organic solvent;
a first reaction container, connected to the syringe and loaded with the sample;
a second reaction container, connected to the first reaction container and loaded with second organic solvent; and
a plurality of fluorescent substances, dispersed in the second organic solvent,
wherein when the target in the sample is extracted by the first organic solvent and reacts with the fluorescent substances in the second organic solvent, the fluorescence emitted by the fluorescent substances is quenched,
wherein the target comprises a synthetic cannabis drug, and the fluorescent substances are decanethiol-protected gold nanoclusters.

### Claim 2 (depends on 1)

The detection kit as claimed in claim 1, wherein the first organic solvent comprises toluene, xylene, dimethyl sulfoxide, dimethyl formamide, chlorobenzene, chloroform, or dichloromethane;
wherein the second organic solvent comprises toluene, xylene, dimethyl sulfoxide, dimethyl formamide, chlorobenzene, chloroform, or dichloromethane.

### Claim 3 (depends on 1)

The detection kit as claimed in claim 1, wherein particle sizes of the fluorescent substances range from 1.02 nm to 2.36 nm.

### Claim 4 (depends on 1)

The detection kit as claimed in claim 1, wherein the fluorescence emitted by the fluorescent substances is orange-red fluorescence excited by ultraviolet light irradiation.